Stefanie Nöpel-Dünnebacke, Hendrik Jan Jütte, Karsten Schulmann, Ullrich Graeven, Rainer Porschen, Jan Stöhlmacher, Susanna Hegewisch‑Becker, Arne Peter Raulf, Dirk Arnold, Anke Claudia Reinacher-Schick, Andrea Tannapfel
- \(\bf Introduction\)
In a retrospective analysis of two randomized phase III trials in mCRC patients treated first line with oxaliplatin, fluoropyrimidine with and without Bevacizumab (the AIO KRK 0207 and R091 trials) we evaluated the association of high microsatellite instability (MSI-H), immunoscore (IS) and PD-L1 expression in relation to overall survival (OS).
\(\bf Methods\)
In total, 550 samples were analysed. Immunohistochemical analysis of the MMR proteins and additionally fragment length analysis was performed, molecular examinations via allele-discriminating PCR in combination with DNA sequencing. Furthermore PD-L1 and IS were assessed.
\(\bf Results\)
MSI-H tumors were more frequent in right sided tumors (13.66% vs. 4.14%) and were correlated with mutant BRAF (\(\it p\) = 0.0032), but not with KRAS nor NRAS mutations (MT). 3.1% samples were found to be PD-L1 positive, there was no correlation of PDL1 expression with MSI-H status, but in a subgroup analysis of MSI-H tumors the percentage of PD-L1 positive tumors was higher than in MSS tumors (9.75% vs. 2.55%). 8.5% of samples showed a positive IS, MSI-H was associated with a high IS. The mean IS of the pooled population was 0.57 (SD 0.97), while the IS of MSI-H tumors was significantly higher (mean of 2.4; SD 1.4; \(\it p\) =< 0.0001).
\(\bf Discussion\)
Regarding OS in correlation with MSI-H, PD-L1 and IS status we did not find a significant difference. However, PD-L1 positive mCRC tended to exhibit a longer OS compared to PD-L1 negative cancers (28.9 vs. 22.1 months).
MetadatenAuthor: | Stefanie Nöpel-DünnebackeORCiDGND, Hendrik Jan JütteGND, Karsten SchulmannGND, Ullrich GraevenGND, Rainer PorschenGND, Jan StöhlmacherGND, Susanna Hegewisch‑BeckerGND, Arne Peter RaulfGND, Dirk ArnoldORCiDGND, Anke Claudia Reinacher-SchickORCiDGND, Andrea TannapfelORCiDGND |
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URN: | urn:nbn:de:hbz:294-97325 |
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DOI: | https://doi.org/10.1007/s00432-021-03559-w |
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Parent Title (English): | Journal of cancer research and clinical oncology |
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Subtitle (English): | a pooled analysis of the AIO KRK 0207 and RO91 trials |
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Publisher: | Springer |
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Place of publication: | Berlin |
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Document Type: | Article |
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Language: | English |
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Date of Publication (online): | 2023/03/03 |
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Date of first Publication: | 2021/03/06 |
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Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
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Tag: | Bevacizumab; Immunoscore; Metastatic colorectal cancer; Microsatellite instability; Overall survival; PD-L1 |
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Volume: | 147 |
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First Page: | 3063 |
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Last Page: | 3072 |
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Note: | Dieser Beitrag ist auf Grund des DEAL-Springer-Vertrages frei zugänglich. |
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Institutes/Facilities: | Institut für Pathologie |
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Dewey Decimal Classification: | Technik, Medizin, angewandte Wissenschaften / Medizin, Gesundheit |
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open_access (DINI-Set): | open_access |
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Licence (English): | Creative Commons - CC BY 4.0 - Attribution 4.0 International |
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