\(\textit {Chlamydomonas reinhardtii}\) mutants deficient for Old Yellow Enzyme 3 exhibit increased photooxidative stress
- Old Yellow Enzymes (OYEs) are flavin-containing ene-reductases that have been intensely studied with regard to their biotechnological potential for sustainable chemical syntheses. OYE-encoding genes are found throughout the domains of life, but their physiological role is mostly unknown, one reason for this being the promiscuity of most ene-reductases studied to date. The unicellular green alga \(\textit {Chlamydomonas reinhardtii}\) possesses four genes coding for OYEs, three of which we have analyzed biochemically before. Ene-reductase CrOYE3 stood out in that it showed an unusually narrow substrate scope and converted \(\it N\)-methylmaleimide (NMI) with high rates. This was recapitulated in a \(\textit {C. reinhardtii croye3}\) mutant that, in contrast to the wild type, hardly degraded externally added NMI. Here we show that CrOYE3-mediated NMI conversion depends on electrons generated photosynthetically by photosystem II (PSII) and that the \(\it croye3\) mutant exhibits slightly decreased photochemical quenching in high light. Non-photochemical quenching is strongly impaired in this mutant, and it shows enhanced oxidative stress. The phenotypes of the mutant suggest that \(\textit {C. reinhardtii}\) CrOYE3 is involved in the protection against photooxidative stress, possibly by converting reactive carbonyl species derived from lipid peroxides or maleimides from tetrapyrrole degradation.
Author: | Stefanie BöhmerORCiDGND, Christina Maria MarxORCiDGND, Reimund GossORCiDGND, Matthias GilbertGND, Severin SassoORCiD, Thomas HappeORCiDGND, Anja Christine HemschemeierORCiDGND |
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URN: | urn:nbn:de:hbz:294-104942 |
DOI: | https://doi.org/10.1002/pld3.480 |
Parent Title (English): | Plant direct |
Publisher: | Wiley |
Place of publication: | Hoboken |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2023/12/01 |
Date of first Publication: | 2023/01/15 |
Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
Tag: | Open Access Fonds |
Volume: | 7 |
Issue: | 1, Article e480 |
First Page: | e480-1 |
Last Page: | e480-14 |
Note: | Article Processing Charge funded by the Deutsche Forschungsgemeinschaft (DFG) and the Open Access Publication Fund of Ruhr-Universität Bochum. |
Institutes/Facilities: | Fakultät für Biologie und Biotechnologie, Abteilung für Biochemie der Pflanzen, Arbeitsgruppe Photobiotechnologie |
Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie |
open_access (DINI-Set): | open_access |
faculties: | Fakultät für Biologie und Biotechnologie |
Licence (English): | Creative Commons - CC BY-NC 4.0 - Attribution-NonCommercial 4.0 International |