Cerebral organoids maintain the expression of neural stem cell-associated glycoepitopes and extracellular matrix
- During development, the nervous system with its highly specialized cell types forms from a pool of relatively uniform stem cells. This orchestrated process requires tight regulation. The extracellular matrix (ECM) is a complex network rich in signaling molecules, and therefore, of interest in this context. Distinct carbohydrate structures, bound to ECM molecules like Tenascin C (TNC), are associated with neural stem/progenitor cells. We have analyzed the expression patterns of the LewisX (LeX) trisaccharide motif and of the sulfation-dependent DSD-1 chondroitin sulfate glycosaminoglycan epitope in human cerebral organoids, a 3D model for early central nervous system (CNS) development, immunohistochemically. In early organoids we observed distinct expression patterns of the glycoepitopes, associated with rosette-like structures that resemble the neural tube in vitro: Terminal LeX motifs, recognized by the monoclonal antibody (mAb) \(487^{LeX}\), were enriched in the lumen and at the outer border of neural rosettes. In contrast, internal LeX motif repeats detected with mAb \(5750^{LeX}\) were concentrated near the lumen. The DSD-1 epitope, labeled with mAb 473HD, was detectable at rosette borders and in adjacent cells. The epitope expression was maintained in older organoids but appeared more diffuse. The differential glycoepitope expression suggests a specific function in the developing human CNS.
Author: | Lars RollGND, Katrin LessmannGND, Oliver BrüstleGND, Andreas FaissnerORCiDGND |
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URN: | urn:nbn:de:hbz:294-88793 |
DOI: | https://doi.org/10.3390/cells11050760 |
Parent Title (English): | Cells |
Publisher: | MDPI |
Place of publication: | Basel |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2022/05/02 |
Date of first Publication: | 2022/02/22 |
Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
Tag: | DSD-1 epitope; LewisX epitope; PTPRZ1; Tenascin C; cerebral organoid; chondroitin sulfate; extracellular matrix; glycoepitope; neural stem cell; radial glia |
Volume: | 11 |
Issue: | 5, Article 760 |
First Page: | 760-1 |
Last Page: | 760-27 |
Institutes/Facilities: | Lehrstuhl für Zellmorphologie und Molekulare Neurobiologie |
Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie |
open_access (DINI-Set): | open_access |
faculties: | Fakultät für Biologie und Biotechnologie |
Licence (English): | Creative Commons - CC BY 4.0 - Attribution 4.0 International |