Scan, extract, wrap, compute — a 3D method to analyse morphological shape differences
- Quantitative analysis of shape and form is critical in many biological disciplines, as context-dependent morphotypes reflect changes in gene expression and physiology, e.g., in comparisons of environment-dependent phenotypes, forward/reverse genetic assays or shape development during ontogenesis. 3D-shape rendering methods produce models with arbitrarily numbered, and therefore non-comparable, mesh points. However, this prevents direct comparisons. We introduce a workflow that allows the generation of comparable 3D models based on several specimens. Translocations between points of modelled morphotypes are plotted as heat maps and statistically tested. With this workflow, we are able to detect, model and investigate the significance of shape and form alterations in all spatial dimensions, demonstrated with different morphotypes of the pond-dwelling microcrustacean Daphnia. Furthermore, it allows the detection even of inconspicuous morphological features that can be exported to programs for subsequent analysis, e.g., streamline- or finite-element analysis.
| Author: | Martin HorstmannORCiDGND, Alexander T. TophamGND, Petra StammGND, Sebastian KruppertORCiDGND, John K. ColbourneGND, Ralph TollrianORCiDGND, Linda C. WeissORCiDGND |
|---|---|
| URN: | urn:nbn:de:hbz:294-88517 |
| DOI: | https://doi.org/10.7717/peerj.4861 |
| Parent Title (English): | PeerJ |
| Publisher: | PeerJ |
| Place of publication: | San Diego |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2022/04/25 |
| Date of first Publication: | 2018/06/08 |
| Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
| Tag: | Open Access Fonds 3D morphological comparison; 3D morphology; Confidence ellipsoids; Confocal microscopy; Daphnia; Landmark-rare shapes; Shape and form analysis; Visualisation of shape alteration |
| Volume: | 6 |
| Issue: | Artikel e4861 |
| First Page: | e4861-1 |
| Last Page: | e4861-20 |
| Note: | Article Processing Charge funded by the Deutsche Forschungsgemeinschaft (DFG) and the Open Access Publication Fund of Ruhr-Universität Bochum. |
| Institutes/Facilities: | Lehrstuhl für Evolutionsökologie und Biodiversität der Tiere |
| Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie |
| open_access (DINI-Set): | open_access |
| faculties: | Fakultät für Biologie und Biotechnologie |
| Licence (English): |  Creative Commons - CC BY 4.0 - Attribution 4.0 International |
