RUB » Bibliotheksportal

Carlos Plaza Sirvent, Bei Zhao, Alisha W. Bronietzki, Marina C. Pils, Neda Tafrishi, Marc Schuster, Till Strowig, Ingo Schmitz

• Autophagy is an evolutionary conserved catabolic pathway that ensures the degradation of intracellular components. The autophagic pathway is regulated by autophagy-related (Atg) proteins that govern formation of double-membraned vesicles called autophagosomes. Autophagy deficiency in regulatory T (Treg) cells leads to increased apoptosis of these cells and to the development of autoimmune disorders, predominantly characterized by intestinal inflammation. Recently, ROR\(\gamma\)t-expressing Treg cells have been identified as key regulators of gut homeostasis, preventing intestinal immunopathology. To study the role of autophagy in ROR\(\gamma\)t\(^{+}\) Foxp3\(^{+}\) Treg cells, we generated mice lacking the essential component of the core autophagy machinery Atg5 in Foxp3\(^{+}\) cells. Atg5 deficiency in Treg cells led to a predominant intestinal inflammation. While Atg5-deficient Treg cells were reduced in peripheral lymphoid organs, the intestinal ROR\(\gamma\)t\(^{+}\) Foxp3\(^{+}\) subpopulation of Treg cells was most severely affected. Our data indicated that autophagy is essential to maintain the intestinal ROR\(\gamma\)t\(^{+}\) Foxp3\(^{+}\) Treg population, thereby protecting the mice from gut inflammatory disorders.