Functional characterization of olfactory receptors in the thyroid gland
- Olfactory receptors (ORs) are almost ubiquitously expressed in the human body. However, information about their functions in these tissues is lacking. To date, no functional characterization of expressed ORs in the human thyroid has been performed. In this study, we detected and compared the expression of OR2H2 and OR2W3 in healthy and malignant cell lines and their corresponding tissues, respectively. We demonstrated that stimulation of ORs by their specific ligand resulted in a transient increase in intracellular calcium and cAMP concentrations. In the case of OR2H2, the downstream signaling cascade analysis revealed that adenylate cyclase (AC) and phosphoinositide phospholipase C (PLC) were involved. Furthermore, OR2H2 and OR2W3 activation affected migration, proliferation, and invasion. These are the first insights that ORs influence physiology-relevant processes in the healthy and malignant thyroid.
| Author: | Daniel WeidingerGND, Nikolina JovancevicGND, Denise ZwanzigerORCiDGND, Sarah TheurerGND, Judith HönesGND, Dagmar FührerGND, Hanns HattGND |
|---|---|
| URN: | urn:nbn:de:hbz:294-87682 |
| DOI: | https://doi.org/10.3389/fphys.2021.676907 |
| Parent Title (English): | Frontiers in physiology |
| Publisher: | Frontiers |
| Place of publication: | Lausanne |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2022/03/24 |
| Date of first Publication: | 2021/07/27 |
| Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
| Tag: | Open Access Fonds CRISPR/Cas9; G protein-coupled receptor; calcium imaging; olfactory receptor; thyroid cancer |
| Volume: | 12 |
| Issue: | Article 676907 |
| First Page: | 676907-1 |
| Last Page: | 676907-12 |
| Note: | Article Processing Charge funded by the Open Access Publication Fund of Ruhr-Universität Bochum. |
| Institutes/Facilities: | Lehrstuhl für Zellphysiologie |
| Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie |
| open_access (DINI-Set): | open_access |
| Licence (English): |  Creative Commons - CC BY 4.0 - Attribution 4.0 International |
