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Alison K. Bauer, Katelyn J. Siegrist, Melanie Wolff, Lindsey Nield, Thomas Brüning, Brad L. Upham, Heiko Käfferlein, Sabine Plöttner

• The WHO classified air pollution as a human lung carcinogen and polycyclic aromatic hydrocarbons (PAHs) are components of both indoor (e.g., tobacco smoke and cookstoves) and outdoor (e.g., wildfires and industrial and vehicle emissions) air pollution, thus a human health concern. However, few studies have evaluated the adverse effects of low molecular weight (LMW) PAHs, the most abundant PAHs in the environment. We hypothesized that LMW PAHs combined with the carcinogenic PAH benzo[\(\it a\)]pyrene (B[\(\it a\)]P) act as co-carcinogens in human lung epithelial cell lines (BEAS-2B and A549). Therefore, in this paper, we evaluate several endpoints, such as micronuclei, gap junctional intercellular communication (GJIC) activity, cell cycle analysis, \(\it anti\)-BPDE-DNA adduct formation, and cytotoxicity after mixed exposures of LMW PAHs with B[\(\it a\)]P. The individual PAH doses used for each endpoint did not elicit cytotoxicity nor cell death and were relevant to human exposures. The addition of a binary mixture of LMW PAHs (fluoranthene and 1-methylanthracene) to B[\(\it a\)]P treated cells resulted in significant increases in micronuclei formation, dysregulation of GJIC, and changes in cell cycle as compared to cells treated with either B[\(\it a\)]P or the binary mixture alone. In addition, \(\it anti\)-BPDE-DNA adducts were significantly increased in human lung cells treated with B[\(\it a\)]P combined with the binary mixture of LMW PAHs as compared to cells treated with B[\(\it a\)]P alone, further supporting the increased co-carcinogenic potential by LMW PAHs. Collectively, these novel studies using LMW PAHs provide evidence of adverse pulmonary effects that should warrant further investigation.