Characterization of two hydrogen peroxide resistant peroxidases from \(\textit {Rhodococcus opacus}\) 1CP
- The dye-decolorizing peroxidases (DyP) are a family of heme-dependent enzymes present on a broad spectrum of microorganisms. While the natural function of these enzymes is not fully understood, their capacity to degrade highly contaminant pigments such as azo dyes or anthraquinones make them excellent candidates for applications in bioremediation and organic synthesis. In this work, two novel DyP peroxidases from the organism \(\textit {Rhodococcus opacus}\) 1CP (DypA and DypB) were cloned and expressed in \(\textit {Escherichia coli}\). The enzymes were purified and biochemically characterized. The activities of the two DyPs via 2,2'-azino-bis [3-ethylbenzthiazoline-6-sulphonic acid] (ABTS) assay and against Reactive Blue 5 were assessed and optimized. Results showed varying trends for DypA and DypB. Remarkably, these enzymes presented a particularly high tolerance towards \(H_{2}O_{2}\), retaining its activities at about 10 mM \(H_{2}O_{2}\) for DypA and about 4.9 mM \(H_{2}O_{2}\) for DypB.
| Author: | Anna Christina R. NgoGND, Catleen ConradGND, Álvaro Gómez BaraibarORCiDGND, Anke MaturaGND, Karl-Heinz van PéeGND, Dirk TischlerORCiDGND |
|---|---|
| URN: | urn:nbn:de:hbz:294-85311 |
| DOI: | https://doi.org/10.3390/app11177941 |
| Parent Title (English): | Applied sciences |
| Publisher: | MDPI |
| Place of publication: | Basel |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2022/01/14 |
| Date of first Publication: | 2021/08/27 |
| Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
| Tag: | Actionbacteria; Dyp-type peroxidase; \(H_{2}O_{2}\) tolerance; biotransformation; dye degradation; heme-dependent enzyme |
| Volume: | 11 |
| Issue: | 17, Article 7941 |
| First Page: | 7941-1 |
| Last Page: | 7941-19 |
| Institutes/Facilities: | Nachwuchsgruppe Mikrobielle Biotechnologie |
| Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie |
| open_access (DINI-Set): | open_access |
| faculties: | Fakultät für Biologie und Biotechnologie |
| Licence (English): |  Creative Commons - CC BY 4.0 - Attribution 4.0 International |
