SecY-mediated quality control prevents the translocation of non-gated porins
- OmpC and OmpF are among the most abundant outer membrane proteins in \(\text it {E. coli}\) and serve as hydrophilic channels to mediate uptake of small molecules including antibiotics. Influx selectivity is controlled by the so-called constriction zone or eyelet of the channel. Mutations in the loop domain forming the eyelet can disrupt transport selectivity and thereby interfere with bacterial viability. In this study we show that a highly conserved motif of five negatively charged amino acids in the eyelet, which is critical to regulate pore selectivity, is also required for SecY-mediated transport of OmpC and OmpF into the periplasm. Variants with a deleted or mutated motif were expressed in the cytosol and translocation was initiated. However, after signal peptide cleavage, import into the periplasm was aborted and the mutated proteins were redirected to the cytosol. Strikingly, reducing the proof-reading capacity of SecY by introducing the PrlA4 substitutions restored transport of OmpC with a mutated channel domain into the periplasm. Our study identified a SecY-mediated quality control pathway to restrict transport of outer membrane porin proteins with a deregulated channel activity into the periplasm.
Author: | Sebastian JungORCiDGND, Verian BaderORCiDGND, Ana NatriashviliGND, Hans-Georg KochORCiDGND, Konstanze WinklhoferORCiDGND, Jörg TatzeltORCiDGND |
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URN: | urn:nbn:de:hbz:294-81409 |
DOI: | https://doi.org/10.1038/s41598-020-73185-y |
Parent Title (English): | Scientific reports |
Publisher: | Springer Nature |
Place of publication: | Berlin |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2021/06/10 |
Date of first Publication: | 2020/10/01 |
Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
Tag: | Open Access Fonds Cell biology; Molecular biology |
Volume: | 10 |
Issue: | Artikel 16347 |
First Page: | 16347-1 |
Last Page: | 16347-13 |
Note: | Article Processing Charge funded by the Deutsche Forschungsgemeinschaft (DFG) and the Open Access Publication Fund of Ruhr-Universität Bochum. |
Institutes/Facilities: | Institut für Biochemie und Pathobiochemie, Abteilung Biochemie Neurodegenerativer Erkrankungen |
Institut für Biochemie und Pathobiochemie, Abteilung für Molekulare Zellbiologie | |
open_access (DINI-Set): | open_access |
faculties: | Medizinische Fakultät |
Licence (English): | Creative Commons - CC BY 4.0 - Attribution 4.0 International |