CalibraCurve: a tool for calibration of targeted MS-based measurements
- Targeted proteomics techniques allow accurate quantitative measurements of analytes in complex matrices with dynamic linear ranges that span up to 4–5 orders of magnitude. Hence, targeted methods are promising for the development of robust protein assays in several sensitive areas, for example, in health care. However, exploiting the full method potential requires reliable determination of the dynamic range along with related quantification limits for each analyte. Here, a software named CalibraCurve that enables an automated batch-mode determination of dynamic linear ranges and quantification limits for both targeted proteomics and similar assays is presented. The software uses a variety of measures to assess the accuracy of the calibration, namely precision and trueness. Two different kinds of customizable graphs are created (calibration curves and response factor plots). The accuracy measures and the graphs offer an intuitive, detailed, and reliable opportunity to assess the quality of the model fit. Thus, CalibraCurve is deemed a highly useful and flexible tool to facilitate the development and control of reliable SRM/MRM-MS-based proteomics assays.
Author: | Michael KohlORCiDGND, Markus StepathGND, Thilo BrachtORCiDGND, Dominik Andre MeggerGND, Barbara SitekGND, Katrin Marcus-AlicORCiDGND, Martin EisenacherORCiDGND |
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URN: | urn:nbn:de:hbz:294-81360 |
DOI: | https://doi.org/10.1002/pmic.201900143 |
Parent Title (English): | Proteomics |
Publisher: | Wiley-VCH Verlag |
Place of publication: | Weinheim |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2021/06/09 |
Date of first Publication: | 2020/02/22 |
Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
Tag: | Calibration; Limit of quantification; Linear range; Multiple-reaction monitoring; Targeted proteomics |
Volume: | 20 |
Issue: | 11, Artikel 1900143 |
First Page: | 1900143-1 |
Last Page: | 1900143-6 |
Note: | Dieser Beitrag ist auf Grund des DEAL-Wiley-Vertrages frei zugänglich. |
Institutes/Facilities: | Medizinisches Proteom-Center |
Dewey Decimal Classification: | Technik, Medizin, angewandte Wissenschaften / Medizin, Gesundheit |
open_access (DINI-Set): | open_access |
faculties: | Medizinische Fakultät |
Licence (English): | Creative Commons - CC BY 4.0 - Attribution 4.0 International |