Fluidity and lipid composition of membranes of peroxisomes, mitochondria and the ER from oleic acid-induced \(\textit {Saccharomyces cerevisiae}\)
- The maintenance of a fluid lipid bilayer is key for organelle function and cell viability. Given the critical role of lipid compositions in determining membrane properties and organelle identity, it is clear that cells must have elaborate mechanism for membrane maintenance during adaptive responses to environmental conditions. Emphasis of the presented study is on peroxisomes, oleic acid-inducible organelles that are essential for the growth of yeast under conditions of oleic acid as single carbon source. Here, we isolated peroxisomes, mitochondria and ER from oleic acid-induced \(\textit {Saccharomyces cerevisiae}\) and determined the lipid composition of their membranes using shotgun lipidomics and compared it to lipid ordering using fluorescence microscopy. In comparison to mitochondrial and ER membranes, the peroxisomal membranes were slightly more disordered and characterized by a distinct enrichment of phosphaditylinositol, indicating an important role of this phospholipid in peroxisomal membrane associated processes.
| Author: | Katharina ReglinskiORCiDGND, Laura Louisa SteinfortGND, Erdinc SezginGND, Christian KloseGND, Harald W. PlattaGND, Wolfgang GirzalskyGND, Christian EggelingGND, Ralf ErdmannORCiDGND |
|---|---|
| URN: | urn:nbn:de:hbz:294-78804 |
| DOI: | https://doi.org/10.3389/fcell.2020.574363 |
| Parent Title (English): | Frontiers in cell and developmentag biology |
| Publisher: | Frontiers Media |
| Place of publication: | Lausanne |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2021/02/16 |
| Date of first Publication: | 2020/10/29 |
| Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
| Tag: | Open Access Fonds ER; lipidomics; mitochondria; peroxin; peroxisomes |
| Volume: | 8 |
| Issue: | Artikel 574363 |
| First Page: | 574363-1 |
| Last Page: | 574363-12 |
| Note: | Article Processing Charge funded by the Deutsche Forschungsgemeinschaft (DFG) and the Open Access Publication Fund of Ruhr-Universität Bochum. |
| Institutes/Facilities: | Institut für Biochemie und Pathobiochemie, Abteilung für Systembiochemie |
| Institut für Biochemie und Pathobiochemie, Abteilung Biochemie intrazellulärer Transportprozesse | |
| Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie |
| open_access (DINI-Set): | open_access |
| Licence (English): |  Creative Commons - CC BY 4.0 - Attribution 4.0 International |
