Investigation of olfactory receptor desensitization
- The sense of smell detects and discriminates among thousands of volatile substances using a remarkably large subfamily of G-protein coupled receptors (GPCRs). The characterization of this very important family of GPCRs was hindered for over a decade by the inability to obtain heterologous olfactory receptor (OR) expression. We found that Hsc70t, a testis-enriched variant of the Hsp70 family of heat shock proteins is also present in the olfactory epithelium. Hsc70t coexpression significantly increased the amount of heterologous cells functionally expressing ORs. This study provides the first insight into ORs endocytosis and desensitization by showing that ORs bind \(\beta\)-arrestin2 with high affinity and are internalized via a clathrin-dependent mechanism. Moreover, a novel way of \(\beta\)-arrestin2/OR interaction was discovered in spermatozoa, where hOR17-4 induced nuclear accumulation of \(\beta\)-arrestin2. Clearly, ORs can now be added to the long list of GPCRs that utilize \(\beta\)-arrestins as their significant interaction partners.
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| Author: | Anastasia MashukovaGND |
|---|---|
| URN: | urn:nbn:de:hbz:294-17250 |
| Referee: | Hanns HattGND, Andreas FaissnerORCiDGND |
| Document Type: | Doctoral Thesis |
| Language: | English |
| Date of Publication (online): | 2006/09/15 |
| Date of first Publication: | 2006/09/15 |
| Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
| Granting Institution: | Ruhr-Universität Bochum, International Graduate School of Neuroscience |
| Date of final exam: | 2006/07/04 |
| Creating Corporation: | International Graduate School of Neuroscience |
| GND-Keyword: | Geruchswahrnehmung; Rezeptor; Retinales S-Antigen; Desensibilisierung |
| Institutes/Facilities: | Lehrstuhl für Zellphysiologie |
| Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie |
| faculties: | International Graduate School of Neuroscience (IGSN) |
| Licence (German): |  Keine Creative Commons Lizenz - es gelten der Veröffentlichungsvertrag und das deutsche Urheberrecht |
