bHLH transcription factor Math6 antagonizes TGF-\(\beta\) signalling in reprogramming, pluripotency and early cell fate decisions
- The basic helix-loop-helix (bHLH) transcription factor Math6 (Atonal homolog 8; Atoh8) plays a crucial role in a number of cellular processes during embryonic development, iron metabolism and tumorigenesis. We report here on its involvement in cellular reprogramming from fibroblasts to induced pluripotent stem cells, in the maintenance of pluripotency and in early fate decisions during murine development. Loss of Math6 disrupts mesenchymal-to-epithelial transition during reprogramming and primes pluripotent stem cells towards the mesendodermal fate. Math6 can thus be considered a regulator of reprogramming and pluripotent stem cell fate. Additionally, our results demonstrate the involvement of Math6 in SMAD-dependent TGF beta signalling. We furthermore monitor the presence of the Math6 protein during these developmental processes using a newly generated \(\textit {Math6Flag-tag}\) mouse. Taken together, our results suggest that Math6 counteracts TGF beta signalling and, by this, affects the initiating step of cellular reprogramming, as well as the maintenance of pluripotency and early differentiation.
| Author: | Satya Srirama Karthik DivvelaORCiDGND, Patrick NellGND, Markus NapireiORCiDGND, Holm ZaehresORCiDGND, Jiayu ChenGND, Wanda Maria GerdingORCiDGND, Huu Phuc NguyenORCiDGND, Shaorong GaoGND, Beate Brand-SaberiORCiDGND |
|---|---|
| URN: | urn:nbn:de:hbz:294-67593 |
| DOI: | https://doi.org/10.3390/cells8060529 |
| Parent Title (English): | Cells |
| Publisher: | MDPI |
| Place of publication: | Basel |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2019/11/26 |
| Date of first Publication: | 2019/06/02 |
| Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
| Tag: | Open Access Fonds Atoh8; MET; Math6; TGF beta; mesendoderm; pluripotency; primed state; reprogramming |
| Volume: | 8 |
| Issue: | 6, Artikel 529 |
| First Page: | 529-1 |
| Last Page: | 529-18 |
| Note: | Article Processing Charge funded by the Deutsche Forschungsgemeinschaft (DFG) and the Open Access Publication Fund of Ruhr-Universität Bochum. |
| Institutes/Facilities: | Institut für Anatomie, Abteilung für Anatomie und Molekulare Embryologie |
| Dewey Decimal Classification: | Technik, Medizin, angewandte Wissenschaften / Medizin, Gesundheit |
| open_access (DINI-Set): | open_access |
| faculties: | Medizinische Fakultät |
| Licence (English): |  Creative Commons - CC BY 4.0 - Attribution 4.0 International |
