Transcriptional and translational regulation of zebrafish Connexin genes, zfCx55.5 & zfCx52.6
- Gap junction proteins, connexins usually show broad tissue expression. However, two zebrafish connexins, zfCx55.5 and zfCx52.6 show highly restricted expression pattern in the subsets of neurons in the fish retina. In order to get the initial answers to the question of their cell specific expression in horizontal cells, promoter elements of these two connexins were characterised. The efficacy of these promoter elements was confirmed by generating a transgenic fish. Moreover, here we provide an evidence for the unique mechanism of internal translation of carboxy-terminal domain of zfCx55.5 mediated by internal IRES element. Interestingly, our in-vitro and in-vivo data indicate that this internally translated product can translocate to cell nucleus. Two cis-acting elements called polypyrimidine tracts (PPT1 and PPT2) and a trans-acting factor called polypyrimidine tract binding protein (PTB) were found important for the IRES mediated internal translation of carboxy-terminal domain of zfCx55.5.
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| Author: | ul-hussain MahboobGND |
|---|---|
| URN: | urn:nbn:de:hbz:294-13746 |
| Referee: | Rolf DermietzelGND |
| Document Type: | Doctoral Thesis |
| Language: | English |
| Date of Publication (online): | 2005/07/28 |
| Date of first Publication: | 2005/07/28 |
| Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
| Granting Institution: | Ruhr-Universität Bochum, International Graduate School of Neuroscience |
| Date of final exam: | 2005/06/10 |
| Creating Corporation: | International Graduate School of Neuroscience |
| GND-Keyword: | Promotor (Genetik); Ribosonale Bindungsstelle; Zellkern; RNS-Bindungsproteine; Transgene Tiere |
| Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie |
| faculties: | International Graduate School of Neuroscience (IGSN) |
| Licence (German): |  Keine Creative Commons Lizenz - es gelten der Veröffentlichungsvertrag und das deutsche Urheberrecht |
